1x thermopol reaction buffer (New England Biolabs)

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New England Biolabs 1x thermopol reaction buffer
1x Thermopol Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 2334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermopol+reaction+buffer/ThermoPol+Reaction+Buffer/pmc12993374-147-70-74
Average 98 stars, based on 2334 article reviews

1x thermopol reaction buffer - by Bioz Stars, 2026-09

98/100 stars

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Polymerase Chain Reaction:

Article Title: CenIR, an essential BlaIR-family regulatory system in C. difficile
Article Snippet: .. To prepare template DNA for PCR, a single colony is picked into 50 μL ThermoPol reaction buffer (NEB) with 0.5 μL thermolabile ProteinaseK (NEB), incubated at 37°C for 30 min, then the proteinase is heat deactivated for 10 min at 55°C. ..

Incubation:

Recombinant:

Article Title: crRNA scaffold remodeling controls CRISPR-Cas12a activity for enhanced performance.
Article Snippet: .. LbCas12a (Cpf1, 100 μM), NEBuffer r2.1 (1 ×; 50 mM NaCl, 10 mM Tris –HCl, 10 mM MgCl 2 , 100 μg/ml Recombinant Albumin; pH 7.9), the Large Fragment Bst DNA polymerase (8 000 U/ml), ThermoPol ® Reaction Buffer (1 ×; 20 mM Tris –HCl, 10 mM (NH 4 ) 2 SO 4 , 10 mM KCl, 2 mM MgSO 4 , 0.1% Triton ® X100; pH 8.8), MgSO 4 solution (100 mM), dNTP mix, and individual dATP/dGTP/dTTP nucleotides were purchased from New England Biolabs (Beijing, China). .. The DNA isothermal rapid amplification kit was obtained from Amp Future Bio-Tech Co. (Changzhou, China).

Article Title: crRNA scaffold remodeling controls CRISPR–Cas12a activity for enhanced performance
Article Snippet: .. LbCas12a (Cpf1, 100 μM), NEBuffer r2.1 (1×; 50 mM NaCl, 10 mM Tris–HCl, 10 mM MgCl 2 , 100 μg/ml Recombinant Albumin; pH 7.9), the Large Fragment Bst DNA polymerase (8 000 U/ml), ThermoPol ® Reaction Buffer (1×; 20 mM Tris–HCl, 10 mM (NH 4 ) 2 SO 4 , 10 mM KCl, 2 mM MgSO 4 , 0.1% Triton ® X-100; pH 8.8), MgSO 4 solution (100 mM), dNTP mix, and individual dATP/dGTP/dTTP nucleotides were purchased from New England Biolabs (Beijing, China). .. The DNA isothermal rapid amplification kit was obtained from Amp Future Bio-Tech Co. (Changzhou, China).

SYBR Green Assay:

Article Title: Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicron
Article Snippet: .. Briefly, each 20 μl reaction contained 1× Thermopol Reaction Buffer (NEB, Cat. no. B9004S), 125 μM dNTPs (Thermo Fisher Scientific, Cat. no. 10297018), 2.5 mM MgSO 4 (NEB, Cat. no. B1003S), 1X SYBR Green I (Lonza, Cat. no. 50513), 500 nM gene specific or 65 nM 16S rRNA primer specific to Bacteroides species and 0.5 units Hot Start Taq Polymerase (NEB, Cat. no. M0495S). ..

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